My_GIF.py

from tkinter import filedialog,Tk
from PIL import Image

rt=Tk()
rt.withdraw()
path=filedialog.askopenfilenames(title='选择图片文件',filetypes=[('png','.png')],initialdir='./')

img_list = []
for i in path:
    img = Image.open(i)
    img_list.append(img)

img_list[0].save("bot.gif",save_all=True,
            append_images=img_list[1:])