My_GIF.py

from tkinter import filedialog,Tk
from PIL import Image

root = Tk()
root.withdraw()
path = filedialog.askopenfilenames(title='选择图片文件',
                                 filetypes=[('png ','.png')],
                                 initialdir='./')

sequence = []
for i in path:
    img = Image.open(i)
    sequence.append(img)

sequence[0].save("bot.gif", save_all=True,
                append_images=sequence[1:])