My_GIF.py

from tkinter import filedialog,Tk
from PIL import Image

root = Tk()
root.withdraw()
path = filedialog.askopenfilenames(title='选择图片文件',filetypes=[('png ','.png')],initialdir='./')
img_list = []
for i in path:
    img = Image.open(i)
    img_list.insert(0,img)
img_list[0].save("bot.gif", save_all=True,append_images=img_list[1:])
print(img_list)